Microneddle
An efficient and patient-friendly process for administering immunochemotherapy is achieved through microneedle (MN)-mediated drug delivery into the dermal interstitium across the stratum corneum. Herein, we designed an interpenetrating polymer network (IPN) hydrogel to fabricate separable MNs for transdermal codelivery of lipopolysaccharide (LPS) and doxorubicin (DOX) for a synergistic immunochemotherapeutic outcome.
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Microcarriers
The use of microspheres for culturing adherent cells has been proven as an important method, allowing for obtaining adequate number of cells in limited space and volume of medium for the intended cell-based medical applications. However, the use of proteolytic enzymes for cell harvesting from the microsphere resulted in cell damage and loss of functionality. Therefore, in this study, we developed a novel redox/thermo-responsive dissolvable gelatin-based microsphere for successful cell proliferation and harvesting adequate high-quality cells using non-enzymatic cell detachment methods.
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Thermal sensitive Hydrogel
Localized cancer therapy with combination of drugs is an effective treatment modality which increase inhibition of tumor growth and reoccurrence. Recently, hydrogels that can encapsulate more than one drug for local sustain releases have been emerging as a compelling choice. In this study, DOX-loaded DMXAA conjugated mPEG-PLGA micelle (DOX-mPPD) was encapsulated in thermosensitive hydrogel prepared by blending carboxylic and amine terminal PDLLA-PEG-PDLLA copolymers and locally injected into tumor-bearing mice to assess the synergistic anti-vascular and immuno-chemotheraputic effects of DMXAA and DOX.
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Funtional Polymer
In our study, we successfully developed the mPEG-P(LA-DSeDEA)-PCL copolymer with the pendant selenocystamine group which self-assembled into micelles in aqueous solutions. Visible light-induced in situ diselenide metathesis in the interface between the hydrophobic and hydrophilic regions of NCMs resulted in the formation of CCMs. The micelles demonstrated appreciable drug loading capacities, and DOX-loaded micelles can undergo redox stimuli triggered release of DOX selectively in cancer cells, making the micelles appropriate for DDAs. The CCMs unveiled extended colloidal stability in the presence of BSA and retained their micellar structure in the presence of 10-fold DMF, unlike their noncross-linked counterparts. These findings suggest that CCMs could be used for the in vivo delivery and controlled release of DOX in tumor tissue. Overall, we developed in situ crosslinked mPEG-P(LA-DSeDEA)-PCL aggregates which maintained their micellar architecture even at extreme dilutions; making CCMs desirable as nanocarriers for intravenous administration of payloads.
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Covalent Organic Framework
A novel thioether-terminated triazole bridge-containing covalent organic framework (TCOF) was constructed via a simple click chemistry between alkyne and azide monomers for dual-sensitive [pH and glutathione (GSH)] anticancer drug delivery systems. The synthesized TCOFs were crystalline in nature with a pore size of approximately 10–30 nm, as confirmed by powder X-ray diffraction spectroscopy and Brunauer–Emmett–Teller technique. Owing to the flexible nature of the synthesized COF, polyethylene glycol (PEG) modification was easily performed to yield a stable TCOF (TCOF-PEG) colloidal solution. Finally, we demonstrated the utility of TCOF-PEG as an in vitro drug delivery system in HeLa cells. TCOF-DOX-PEG exhibited time-dependent release of DOX followed by internalization. Thus, the novel TCOF system reported here opens a new window in COF research for sensitive drug carrier systems.
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